Provided are a method for manufacturing a polymer electrolyte membrane for a fuel cell, a membrane electrode assembly and a polymer electrolyte membrane fuel cell. The manufacturing method includes (S1) forming a plurality of grooves on a substrate; (S2) casting an ion exchange resin solution for forming an electrolyte membrane on a surface of the substrate having the plurality of grooves; (S3) drying the ion exchange resin solution to form a polymer electrolyte membrane; and (S4) separating the formed polymer electrolyte membrane from the substrate. The ion conductive electrolyte membrane manufactured by the method has the grooves formed on the surface thereof, so that a contact area with a catalyst is maximized to improve the performance of the fuel cell, and adhesion of electrodes and the electrolyte membrane is improved to enhance the interface stability with the electrodes.